Biofilm/Mat Assays for Budding Yeast: Figure 1.

RNAi in budding yeast.

RNA interference (RNAi), a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast Saccharomyces cerevisiae. Here, we show that RNAi is present in other budding yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate small interfering ...

Figures and figure supplements Snf 1 / AMPK promotes the formation of Kog 1 / Raptor - bodies to increase the activation threshold of TORC 1 in budding yeast

Imaging Polarization in Budding Yeast.

We describe methods for live-cell imaging of yeast cells that we have exploited to image yeast polarity establishment. As a rare event occurring on a fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By synchronizing cells in a way that increases resistance to photodamage, we discovered unexpected ...

The budding yeast endocytic pathway.

Recent live-cell imaging studies, coupled with powerful genetic, biochemical and pharmacological tests of function, have expanded our understanding of the molecular events that underlie clathrin/actin mediated-endocytosis in budding yeast. Many features of this pathway are evolutionarily conserved (Engqvist-Goldstein and Drubin, 2003; Kaksonen et al, 2006). Therefore, insights into the intricat...

Natural history of budding yeast

The microbe humans love best is yeast. For thousands of years it has provided our favourite drug, alcohol, and in return we have fed and housed it. Most of our long love affair has been conducted in the dark, but in 1875 a Danish brewer founded the Carlsberg Laboratory to apply science to the brewing industry, and since then we have pored over the inner workings of Saccharomyces in a frenzy of ...